Human Endoglycan/PODXL2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1524
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2
Gly33-Thr500
Accession # Q9NZ53
Gly33-Thr500
Accession # Q9NZ53
Specificity
Detects human Endoglycan/PODXL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human PODXL.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Endoglycan/PODXL2 Antibody
Endoglycan/PODXL2 in Human HUVEC.
Endoglycan/PODXL2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Endoglycan/PODXL2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells
Sample: HUVEC human umbilical vein endothelial cells
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human Endoglycan/PODXL2
Sample: Recombinant Human Endoglycan/PODXL2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglycan/PODXL2
Alternate Names
PODLX2, PODXL2
Gene Symbol
PODXL2
UniProt
Additional Endoglycan/PODXL2 Products
Product Documents for Human Endoglycan/PODXL2 Antibody
Product Specific Notices for Human Endoglycan/PODXL2 Antibody
For research use only
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