Human EPCR Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2245

R&D Systems, part of Bio-Techne
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AF2245
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

ELISA Detection, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all EPCR Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EPCR
Ser18-Ser210
Accession # Q9UNN8

Specificity

Detects human EPCR in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse EPCR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human EPCR Biotinylated Antibody

EPCR antibody in Human Liver by Immunohistochemistry (IHC-P).

EPCR in Human Liver.

EPCR was detected in immersion fixed paraffin-embedded sections of human liver array using Goat Anti-Human EPCR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2245) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human EPCR Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

0.1 µg/mL
Sample: Recombinant Human EPCR
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EPCR

Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Long Name

Endothelial Protein C Receptor

Alternate Names

CCD41, CD201, PROCR

Entrez Gene IDs

10544 (Human); 19124 (Mouse)

Gene Symbol

PROCR

UniProt

Q9UNN8

Additional EPCR Products

Product Documents for Human EPCR Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EPCR Biotinylated Antibody

For research use only

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