Human Ephrin-B3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB395

R&D Systems, part of Bio-Techne
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MAB395
MAB395-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 88838

View all Ephrin-B3 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Ephrin-B3
Leu28-Ser224
Accession # Q15768

Specificity

Detects human Ephrin-B3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, rmEphrin-A2, recombinant human (rh) Ephrin-A3, rhEphrin-A4, rhEphrin-A5, rmEphrin-B1, and rmEphrin-B2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Ephrin-B3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Ephrin-B3 Fc Chimera (Catalog # 395-EB)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB395 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-B3

Ephrin-B3, also known as NLERK-2, Elk-L3, EFL-6, ELF-3 and LERK-8 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can be tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 aa long and are highly conserved, especially the last 33 aa. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B3 has been shown to bind Ephrin-A4, Ephrin-B1, Ephrin-B2, and Ephrin-B3 (2, 3). The extracellular domains of murine and human Ephrin-B3 share 98% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter]. Cell (1997) 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Alternate Names

EFL-6, Efnb3, Elk-L3, EphrinB3, Epl8, LERK-8, NLERK-2

Entrez Gene IDs

1949 (Human); 13643 (Mouse)

Gene Symbol

EFNB3

UniProt

Q15768

Additional Ephrin-B3 Products

Product Documents for Human Ephrin-B3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Ephrin-B3 Antibody

For research use only

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