Human EVI-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7506
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 702319
Product Specifications
Immunogen
E. coli-derived recombinant human EVI-1
Gly241-Met430
Accession # Q03112
Gly241-Met430
Accession # Q03112
Specificity
Detects human EVI-1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human EVI-1 Antibody
Detection of Human EVI‑1 by Western Blot.
Western blot shows lysates of MDA-MB-231 human breast cancer cell line and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EVI-1 Monoclonal Antibody (Catalog # MAB7506) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EVI-1 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human EVI-1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: MDA‑MB‑231 human breast cancer cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line
Sample: MDA‑MB‑231 human breast cancer cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EVI-1
Long Name
Ecotropic Viral Integration Site 1
Alternate Names
EVI1, MDS1, MECOM, PRDM3
Gene Symbol
MECOM
UniProt
Additional EVI-1 Products
Product Documents for Human EVI-1 Antibody
Product Specific Notices for Human EVI-1 Antibody
For research use only
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