Human FABP6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3880
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FABP6
Ala2-Ala128
Accession # P51161
Ala2-Ala128
Accession # P51161
Specificity
Detects human FABP6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP7, and recombinant mouse FABP9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human FABP6 Antibody
FABP6 in T84 Human Cell Line.
FABP6 was detected in immersion fixed T84 human colon carcinoma cell line using Goat Anti-Human FABP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human FABP6 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed T84 human colon carcinoma cell line
Sample: Immersion fixed T84 human colon carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human FABP6
Sample: Recombinant Human FABP6
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP6
Fatty acid binding protein-6 (FABP6), also known as ileal bile acid binding protein (I-BABP) and gastrotropin, is a 15 kDa cytoplasmic protein that belongs to the FABP family. It is expressed in ileal epithelium and multiple other tissues. Alternate transcription promoters generate two transcript variants, encoding a 128 aa and a 177 aa residue protein. Human FABP6 isoform 2 contains 128 amino acid residues and is believed to be acetylated on Ala2. It binds both fatty acids and bile acids and has roles in fatty acid transport and metabolism. The amino acid sequence of human FABP6 is 80%, 78%, and 75% aa identical to that of mouse, canine, and porcine FABP6, respectively.
Long Name
Fatty Acid-Binding Protein 6
Alternate Names
Gastrotropin, I-15P, I-BAP, IBABP, ILBP, ILBP3, ILLBP
Gene Symbol
FABP6
UniProt
Additional FABP6 Products
Product Documents for Human FABP6 Antibody
Product Specific Notices for Human FABP6 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...