Human FABP9/T-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5707
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FABP9
Met1-Val132
Accession # Q0Z7S8
Met1-Val132
Accession # Q0Z7S8
Specificity
Detects human FABP9 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse FABP9 and less than 1% cross-reactivity with recombinant human FABP1, 2, 3, 4, 5, 6, 7, and 8 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human FABP9/T-FABP Antibody
FABP9/T‑FABP in Human Testis.
FABP9/T-FABP was detected in immersion fixed paraffin-embedded sections of human testis using Human FABP9/T-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5707) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human FABP9/T-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis
Sample: Immersion fixed paraffin-embedded sections of human testis
Western Blot
0.1 µg/mL
Sample: Recombinant human FABP9
Sample: Recombinant human FABP9
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP9/T-FABP
FABP9 (fatty acid binding protein-9; also T-FABP and PERF15) is a cytosolic 15 kDa member of the fatty acid binding protein family, calycin superfamily of proteins. It is found in midpachytene spermatocytes and round spermatids, and constitutes part of the perinuclear theca. Functionally, FABP9 is likely to link intracellular membranes, and may signal abnormal sperm formation during spermatogenesis. Human FABP9 is 132 amino acids (aa) in length and exhibits two layers of antiparallel beta-strands that envelope a hydrophobic pocket for lipid binding. Human FABP9 shares 71% aa identity with mouse FABP9.
Long Name
Fatty Acid-Binding Protein 9
Alternate Names
PERF 15, T-FABP, TFABP
Gene Symbol
FABP9
UniProt
Additional FABP9/T-FABP Products
Product Documents for Human FABP9/T-FABP Antibody
Product Specific Notices for Human FABP9/T-FABP Antibody
For research use only
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