Human FAM20B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8427

R&D Systems, part of Bio-Techne
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MAB8427-100
MAB8427-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1018512

View all FAM20B Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FAM20B
Asn27-Leu409
Accession # O75063

Specificity

Detects human FAM20B in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FAM20B Antibody

Detection of Human FAM20B antibody by Western Blot.

Detection of Human FAM20B by Western Blot.

Western blot shows lysates of ZR-75 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FAM20B Monoclonal Antibody (Catalog # MAB8427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FAM20B at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FAM20B Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: ZR‑75 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB8427 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAM20B

FAM20B is a member of the FAM20 protein family that has three members in mammals (FAM20A, FAM20B, FAM20C) (1). Human FAM20B shares 97% amino acid sequence identity with mouse and rat FAM20B. FAM20B localizes to the Golgi apparatus where it phosphorylates the xylose residue in the glycosaminoglycan (GAG)-protein linkage region of proteoglycans, which leads to enhanced GAG biosynthesis (2). Accordingly, chondroitin sulfate and heparan sulfate production is increased when FAM20B is over-expressed and reduced following FAM20B knockdown (2). FAM20B knockout mice display embryonic lethality at day E13.5, suggesting that FAM20B has an important role during development (3). Furthermore, in zebrafish, FAM20B mutations result in reduced cartilage matrix production and skeletal defects (4). The enzymatic activity of recombinant human FAM20B is measured using a phosphatase-coupled method (5).

References

  1. Nalbant, D. et al. (2005) BMC Genomics 6:11.
  2. Koike, T. et al. (2009) Biochem. J. 421:157.
  3. Vogel, P. et al. (2012) Vet. Pathol. 49:998.
  4. Eames, B.F. et al. (2011) PLoS Genet. 7:e1002246.
  5. Wu, Z.L. (2011) PLoS One 6:e23172.

Long Name

Family with Sequence Similarity 20, Member B

Alternate Names

Glycosaminoglycan Xylosylkinase, gxk1, XYLK, Xylose Kinase

Entrez Gene IDs

9917 (Human); 215015 (Mouse); 304885 (Rat)

Gene Symbol

FAM20B

UniProt

O75063

Additional FAM20B Products

Product Documents for Human FAM20B Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FAM20B Antibody

For research use only

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