Detects human FCRL1/FcRH1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FCRL2, 3, 4, or 5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human FCRL1/FcRH1 PE-conjugated Antibody

Detection of FCRL1/FcRH1 in Human Blood B cells by Flow Cytometry.

Human blood B cells were stained with Mouse Anti-Human FCRL1/FcRH1 PE-conjugated Monoclonal Antibody (Catalog # FAB2049P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human FCRL1/FcRH1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human blood B cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: FCRL1/FcRH1

Fc Receptor-Like 1 (FCRL1), also known as FcRH1, and IRTA5, is an approximately 50 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL1 binds to purified human IgG. Mature human FCRL1 consists of a 291 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 101 aa cytoplasmic domain with two immunotyrosine activation motifs (ITIMs) (3, 4). A charged glutamic acid within the transmembrane segment may mediate association with other signaling proteins. Alternative splicing may generate an isoform that lacks the transmembrane segment and an isoform that largely consists of the first two Ig-like domains (5). Mouse FCRL1 contains only two Ig-like domains, but it shares 62% aa sequence identity with homologous regions of the human FCRL1 ECD. FCRL1 is expressed on pre-B cells and naive B cells (6, 7). It is down‑regulated upon B cell activation but up‑regulated on memory B cells (6, 8). FCRL1 is expressed on many B cell lymphoma and leukemia tumor cells with the exception of B cell acute lymphoblastic leukemia (9‑11). Antibody crosslinking of FCRL1 triggers its tyrosine phosphorylation and augments B cell proliferation induced by the B cell receptor (6).

References

Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
Miller, I. et al. (2002) Blood 99:2662.
Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
Accession # Q96LA6.
Leu, C.-M. et al. (2005) Blood 105:1121.
Li, F.J. et al. (2008) Blood 112:179.
Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
Du, X. et al. (2008) Blood 111:338.
Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.

Long Name

Fc Receptor-like 1

Alternate Names

BXMH1, CD307a, FcRH1, IFGP1, IRTA5

Entrez Gene IDs

115350 (Human); 229499 (Mouse); 680665 (Rat)

Gene Symbol

FCRL1

UniProt

Q96LA6

Additional FCRL1/FcRH1 Products

Product Documents for Human FCRL1/FcRH1 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FCRL1/FcRH1 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human FCRL1/FcRH1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2049P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications