Detects human FCRL6/FcRH6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) FCRL1, rhFCRL2, rhFCRL3, rhFCRL4, rhFCRL5, rhFcRN, or rhFCRLB/FCRY is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human FCRL6/FcRH6 Antibody

Detection of FCRL6/FcRH6 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human FCRL6/FcRH6 Monoclonal Antibody (Catalog # MAB6745) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Applications for Human FCRL6/FcRH6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL6/FcRH6

FCRL6 (Fc receptor-like protein 6; also FcRH6 and IFGP6) is a 66 kDa member of the Ig Superfamily. It is found on CD56dim CD16⁺ NK cells, CD56⁺ CD3⁺ NKT cells, gd T cells, and effector plus effector-memory CD8⁺ T cells. FCRL6 is not a receptor for immunoglobulin. It does, however, bind to isoforms of HLA-DR that are expressed on APCs. Given that FCRL6 contains a cytoplasmic ITIM, FCRL6 may function as an inhibitory receptor for MHC Class II. Mature human FCRL6 is a 415 amino acid (aa) type I transmembrane protein. It contains a 288 aa extracellular region (aa 20-307) that shows three C2-type Ig-like domains (aa 20-293), and a 106 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 105-200 accompanied by a 13 aa substitution for aa 394-434; a second possesses a 15 aa substitution for aa 383-434; and a third shows an alternative start site seven aa upstream of the standard site accompanied by a 13 aa substitution for aa 394-434. Over aa 16-312, human FCRL6 shares only 33% aa identity with the mouse counterpart to FCRL6.

Long Name

Fc Receptor-like 6

Alternate Names

FcRH6, IFGP6

Entrez Gene IDs

343413 (Human); 677296 (Mouse)

Gene Symbol

FCRL6

UniProt

Q6DN72 (Human)

Additional FCRL6/FcRH6 Products

Product Documents for Human FCRL6/FcRH6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FCRL6/FcRH6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FCRL6/FcRH6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6745

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity