Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 937508

View all FCRN Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FCRN
Ala24-Ser297
Accession # P55899

Specificity

Detects human FCRN in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FCRN Antibody

Detection of Human FCRN antibody by Western Blot.

Detection of Human FCRN by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human FCRN Monoclonal Antibody (Catalog # MAB8639) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FCRN at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of FCRN antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of FCRN in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human FCRN Monoclonal Antibody (Catalog # MAB8639, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human FCRN Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Western Blot

5 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line and HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB8639 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRN

The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells (1, 2). Mature human FCRN consists of a 274 amino acid (aa) extracellular domain (ECD) with two N-terminal alpha domains, one alpha3/immunoglobulin-like domain, a 23 aa transmembrane segment, and a 44 aa cytoplasmic domain (3). Within the ECD, human FCRN shares 68% aa sequence identity with mouse and rat FCRN. Mouse FCRN binds with high affinity to IgG from mouse, human, rat, rabbit, guinea pig, bovine, and sheep, while human FCRN binds IgG with significantly lower affinity and is much more restricted in terms of species recognition (4). It does not bind the structurally related chicken IgY (5). FCRN additionally binds to albumin, and both it and IgG are bound at pH 5.0 but not at pH 8.0 (3, 6). FCRN associates noncovalently with beta 2-Microglobulin, and this interaction is important for the intracellular trafficking of FCRN (7-10). FCRN cycles between the plasma membrane and acidified intracellular compartments of endothelial cells and epithelial cells (5, 8). It binds endocytosed IgG and albumin in the low pH vesicles and transports them to the plasma membrane for extracellular release at higher pH. This protects IgG and albumin from lysosomal degradation and helps maintain the circulating levels of both proteins (5, 6). This mechanism is involved in the bidirectional transport of IgG across epithelial and endothelial barriers including neonatal IgG absorption in the intestine and fetal uptake of maternal antibodies through the placenta (5, 8, 11, 12). In the kidney, FCRN recycles albumin to the serum but removes IgG from the glomular basement membrane and promotes its excretion into the urine (13, 14). FCRN is also expressed in neutrophils and myeloid antigen presenting cells (7, 15, 16). It can enhance IgG-mediated phagocytosis and antigen presentation by these cells, but it promotes the degradation of opsonizing IgG rather than returning it to the circulation (15, 16).

References

  1. Baker, K. et al. (2009) Semin. Immunopathol. 31:223.
  2. Baker, K. et al. (2014) Front. Immunol. 5:408.
  3. Story, C.M. et al. (1994) J. Exp. Med. 180:2377.
  4. Ober, R.J. et al. (2001) Int. Immunol. 13:1551.
  5. Dickinson, B.L. et al. (1999) J. Clin. Invest. 104:903.
  6. Chaudhury, C. et al. (2003) J. Exp. Med. 197:315.
  7. Simister, N.E. and K.E. Mostov (1989) Nature 337:184.
  8. Kobayashi, N. et al. (2002) Am. J. Physiol. Renal Physiol. 282:F358.
  9. Praetor, A. and W. Hunziker (2002) J. Cell Sci. 115:2389.
  10. Zhu, X. et al. (2001) J. Immunol. 166:3266.
  11. Spiekermann, G.M. et al. (2002) J. Exp. Med. 196:303.
  12. Firan, M. et al. (2001) Int. Immunol. 13:993.
  13. Sarav, M. et al. (2009) J. Am. Soc. Nephrol. 20:1941.
  14. Akilesh, S. et al. (2008) Proc. Natl. Acad. Sci. 105:967.
  15. Vidarsson, G. et al. (2006) Blood 108:3573.
  16. Qiao, S.-W. et al. (2008) Proc. Natl. Acad. Sci. 105:9337.

Long Name

Neonatal Fc Receptor

Alternate Names

FCGRT

Entrez Gene IDs

2217 (Human); 14132 (Mouse); 29558 (Rat); 102128913 (Cynomolgus Monkey)

Gene Symbol

FCGRT

UniProt

P55899

Additional FCRN Products

Product Documents for Human FCRN Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FCRN Antibody

For research use only

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