Human Fes Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6460

R&D Systems, part of Bio-Techne
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AF6460
AF6460-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Fes Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Fes
aa 169-370
Accession # P07332

Specificity

Detects human Fes in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Fes Antibody

Detection of Human Fes antibody by Western Blot.

Detection of Human Fes by Western Blot.

Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human Fes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Fes at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Fes Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HEL 92.1.7 human erythroleukemic cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fes

Fes (homolog of feline sarcoma oncogene), also called c-FES or c-FPS, is a 92 kDa protein within subgroup IV of the non-receptor tyrosine kinase family. It is expressed in myeloid hematopoietic cells, vascular endothelium and neurons and regulates cell adhesion and extravasation. The 822 amino acid (aa) human Fes contains F-BAR (aa 7-239), SH2 (aa 460-549) and kinase (aa 561-822) domains. The F-BAR domain contains coiled-coil homo-oligomerization and microtubule binding sequences. Within the region used as an immunogen, human Fes shares 85% and 87% aa identity with mouse and rat Fes, respectively. Human isoforms of 764, 752, 694 and 681 aa may be missing aa 72-129 or 441-511 and/or contain an alternate 17 aa for aa 610-640, while an isoform of 891 aa contains 18 and 51 aa inserted after aa 510 and 568.

Long Name

Feline Sarcoma Proto-oncogene

Alternate Names

FPS

Entrez Gene IDs

2242 (Human); 14159 (Mouse); 361597 (Rat)

Gene Symbol

FES

UniProt

P07332 (Human)

Additional Fes Products

Product Documents for Human Fes Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fes Antibody

For research use only

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