Human FGF-17 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB319
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 121630
Product Specifications
Immunogen
E. coli-derived recombinant human FGF-17
Thr23-Thr216
Accession # O60258
Thr23-Thr216
Accession # O60258
Specificity
Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISA, approximately 7% cross-reactivity with recombinant human (rh) FGF-5 is observed and no cross-reactivity with rhFGF-acidic, rhFGF-basic, rhFGF-4, rhFGF-6, rhFGF-7, rhFGF8b, rhFGF-9, rhFGF-10, rhFGF-17, rhFGF-18, rhFGF-19, recombinant mouse (rm) FGF-8b, rmFGF-8c and rmFGF-15 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FGF-17 Antibody
FGF-17 in Human Kidney.
FGF-17 was detected in immersion fixed paraffin-embedded sections of human kidney using Human FGF-17 Monoclonal Antibody (Catalog # MAB319) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the basement membrane supporting epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human FGF-17 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Western Blot
1 µg/mL
Sample: Recombinant Human FGF-17 (Catalog # 319-FG)
Sample: Recombinant Human FGF-17 (Catalog # 319-FG)
Reviewed Applications
Read 1 review rated 5 using MAB319 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-17
Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30‑70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF‑17 shares 98.6% amino acid (aa) sequence identity with mouse FGF‑17. Rat FGF‑17 shares 100% aa sequence identity with mouse FGF‑17.
References
- Hoshikawa, M. et al. (1998) Biochem. Biophys. Res. Commun. 244:187.
- Xu, J. et al. (1999) Mech. Dev. 83:165.
Long Name
Fibroblast Growth Factor 17
Alternate Names
FGF17
Gene Symbol
FGF17
UniProt
Additional FGF-17 Products
Product Documents for Human FGF-17 Antibody
Product Specific Notices for Human FGF-17 Antibody
For research use only
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