Human Fibulin 5/DANCE Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3095

R&D Systems, part of Bio-Techne
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MAB3095
MAB3095-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 293904

View all Fibulin 5/DANCE Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fibulin 5/DANCE
Gln24-Phe448
Accession # Q9UBX5

Specificity

Detects human Fibulin 5/DANCE in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fibulin 5/DANCE Antibody

Detection of Human Fibulin 5/DANCE antibody by Western Blot.

Detection of Human Fibulin 5/DANCE by Western Blot.

Western blot shows lysates of human heart tissue, human lung tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibulin 5/DANCE Monoclonal Antibody (Catalog # MAB3095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Fibulin 5 (Catalog # 3095-FB) (5 ng/lane) was included. A specific band was detected for Fibulin 5/DANCE at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Fibulin 5/DANCE Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung

Western Blot

1 µg/mL
Sample: Human heart tissue, human lung tissue, and human brain (cerebellum) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4 using MAB3095 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibulin 5/DANCE

Fibulin 5, also known as DANCE and EVEC, is a secreted 55 kDa matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis (1). Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44 amino acid (aa) spacer region, five more tandem EGF-like domains, and a 115 aa Fibulin-like C-terminal region (2, 3). Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5. Fibulin 5 is expressed by smooth muscle cells and endothelial cells of the developing vasculature as well as by migrating neural crest cells and lung interstitial fibroblasts (2‑4). It is down‑regulated in the adult vasculature but is re‑expressed at aortic branching points, in the uterus, and at sites of mechanical or atherosclerotic injury (2, 3, 5). The RGD motif of Fibulin 5 binds to several cell surface Integrins including alphaV beta3, alphaV beta5, alpha9 beta1, alpha4 beta1, and alpha5 beta1 (2, 6, 7). The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix (8). Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2, and the crosslinking lysyl oxidase-like enzymes LOXL1, 2, and 4 along Fibrillin microfibrils (6, 9‑11). In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevents Fibulin 5 from interacting with Fibrillin-1 microfibrils (10). Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation and migration and by limiting vascular sprouting (5, 12). Fibulin 5 is down‑regulated in many malignancies (15). When experimentally re‑expressed, however, Fibulin 5 enhances tumor cell invasiveness and cooperates with TGF-beta in initiating and promoting epithelial-mesenchymal transition (EMT) (13, 15). Defects in Fibulin 5 expression or function can result in a loss of connective tissue integrity, cardiac elasticity, and ability to remodel the vasculature after injury (8, 5, 14).

References

  1. Yanagisawa, H. et al. (2009) J. Cell. Commun. Signal. 3:337.
  2. Nakamura, T. et al. (1999) J. Biol. Chem. 274:22476.
  3. Kowal, R.C. et al. (1999) Circ. Res. 84:1166.
  4. Kuang, P.-P. et al. (2003) Am. J. Physiol. Lung Cell. Mol. Physiol. 285:L1147.
  5. Spencer, J.A. et al. (2005) Proc. Natl. Acad. Sci. 102:2946.
  6. Nakamura, T. et al. (2002) Nature 415:171.
  7. Lomas, A.C. et al. (2007) Biochem. J. 405:417.
  8. Yanagisawa, H. et al. (2002) Nature 415:168.
  9. Wachi, H. et al. (2008) J. Biochem. 143:633.
  10. Hirai, M. et al. (2007) J. Cell Biol. 176:1061.
  11. Hirai, M. et al. (2007) EMBO J. 26:3283.
  12. Sullivan, K.M. et al. (2007) Lab. Invest. 87:818.
  13. Lee, Y.-H. et al. (2008) Carcinogenesis 29:2243.
  14. Loeys, B. et al. (2002) Hum. Mol. Genet. 11:2113.
  15. Schiemann, W.P. et al. (2002) J. Biol. Chem. 277:27367.

Alternate Names

DANCE, EVEC, FBLN5, UP50

Entrez Gene IDs

10516 (Human)

Gene Symbol

FBLN5

UniProt

Q9UBX5

Additional Fibulin 5/DANCE Products

Product Documents for Human Fibulin 5/DANCE Antibody

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Product Specific Notices for Human Fibulin 5/DANCE Antibody

For research use only

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