Human FKBP52 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4095
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 425123
Product Specifications
Immunogen
E. coli-derived recombinant human FKBP52
Met1-Ala459
Accession # Q02790
Met1-Ala459
Accession # Q02790
Specificity
Detects human FKBP52 in Western blots. In Western blots, no cross-reactivity with other FKBP family members is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Human FKBP52 Antibody
Detection of Human FKBP52 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human FKBP52 Monoclonal Antibody (Catalog # MAB4095) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, 5 ng recombinant human FKBP38, FKBP51, and FKBP52 were included. A specific band for FKBP52 was detected at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human FKBP52 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human skin cancer tissue
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line
Sample: MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP52
FK506 binding protein, also called FKBP52 and FKBP4, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans- proline residues critical for proper folding of proteins. It associates with HSP90 complexes that are critical for the proper folding of steroid receptors. FKBP52 knockout mice have abnormal reproductive organ development due to disruption of androgen receptor folding; and phosphorylation of FKBP52 at Thr143 seems important for steroid receptor activity.
Long Name
52 kDa FK506 Binding Protein
Alternate Names
FKBP4, FKBP59, HBI, Hsp56
Gene Symbol
FKBP4
UniProt
Additional FKBP52 Products
Product Documents for Human FKBP52 Antibody
Product Specific Notices for Human FKBP52 Antibody
For research use only
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