Human FoxK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6366
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FoxK1
Lys493-Lys670
Accession # P85037
Lys493-Lys670
Accession # P85037
Specificity
Detects human FoxK1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FoxK1 is observed and less than 5% cross-reactivity with recombinant human (rh) FoxK2 and rhFoxJ1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human FoxK1 Antibody
Detection of Human FoxK1 by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Human FoxK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FoxK1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
FoxK1 in Human Colon.
FoxK1 was detected in immersion fixed paraffin-embedded sections of human colon using Human FoxK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6366) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell nuclei and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human FoxK1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Western Blot
1 µg/mL
Sample: A172 human glioblastoma cell line
Sample: A172 human glioblastoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxK1
FOX K1 (Forkhead box protein K1; also MNF) is a 75 kDa (predicted) nuclear protein that is a Class 1 member of the FOX family of transcription factors. It should not be confused with FOXK2/ILF1 that has also been referred to as FOXK1. It is widely expressed, but has become known as a marker for skeletal muscle satellite cells in the adult. Human FOXK1 is 733 amino acids (aa) in length. It contains one FHA domain that binds phosphopeptides (aa 116-203), plus a forkhead DNA binding region (aa 305-400) and over a dozen Ser/Thr phosphorylation sites. There are multiple potential splice variants. Two utilize an alternative start site at Met118 that may be accompanied by a 28 aa substitution for aa 673-733, while another contains 24 aa substitution for aa 1-187. Over aa 493-670, human FOXK1 shares 90% aa identity with mouse FOXK1.
Long Name
Forkhead Box K1
Alternate Names
MNF
Gene Symbol
FOXK1
UniProt
Additional FoxK1 Products
Product Documents for Human FoxK1 Antibody
Product Specific Notices for Human FoxK1 Antibody
For research use only
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