Human FoxM1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3975
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FoxM1
Thr642-Gln763
Accession # Q08050
Thr642-Gln763
Accession # Q08050
Specificity
Detects human FoxM1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) FoxJ1 is observed and less than 1% cross-reactivity with rhFoxB2, rhFoxC2, rhFoxD3, rhFoxF1, rhFoxH1, rhFoxO3A, rhFoxP1, and rhFoxP3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human FoxM1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human FoxM1
Sample: Recombinant Human FoxM1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxM1
FoxM1 (Forkhead box protein M1; also FKHL16, HFH11 and WIN) is a 90‑100 kDa member of the winged helix transcription factor gene family. It is expressed in proliferating and tumor cells and promotes the transition from G1 to S phase and G2 to M phase within the cell cycle. Human FoxM1 is 763 amino acids (aa) in length. It contains three PEST sequences (aa 22‑34, 460‑472 and 478‑515), a forkhead DNA binding domain (aa 235‑327), a Pro/Ser/Thr-rich region (aa 480‑513) and a phosphorylation site at Ser704. There are at least two splice variants. The first shows a 38 aa insertion after Val423. This blocks FoxM1 transcriptional activation activity. The second variant shows a deletion of aa 326‑340. Over aa 642‑763, human FoxM1 shares 73% aa identity with mouse FoxM1.
Long Name
Forkhead Box M1
Alternate Names
FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP-2, PIG29, Trident
Gene Symbol
FOXM1
UniProt
Additional FoxM1 Products
Product Documents for Human FoxM1 Biotinylated Antibody
Product Specific Notices for Human FoxM1 Biotinylated Antibody
For research use only
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