Human FoxP4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6854
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FoxP4
Pro549-Ser680
Accession # Q8IVH2
Pro549-Ser680
Accession # Q8IVH2
Specificity
Detects human FoxP4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP1, rhFoxP2, rhFoxP3, and rhCTBP1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human FoxP4 Antibody
Detection of Human FoxP4 by Western Blot.
Western blot shows lysates of COS‑7 African green monkey SV40 transformed kidney fibroblast-like cell line either mock transfected, transfected with human FoxP1, transfected with human FoxP2, transfected with human FoxP3, or transfected with human FoxP4. PVDF membrane was probed with 1:1000 dilution of Sheep Anti-Human FoxP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6854) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, the membrane was co-probed with an Anti-Actin Antibody. A specific band was detected for FoxP4 at approximately 75 kDa and Actin at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions.Image courtesy of Dr. Alison Banham, Ms. Linden Lyne, and Dr. Paola Bignone, The University of Oxford, Oxford, UK.Applications for Human FoxP4 Antibody
Application
Recommended Usage
Western Blot
1:1000 dilution
Sample: COS‑7 African green monkey SV40 transformed kidney fibroblast-like cell line transfected with human FoxP4
Sample: COS‑7 African green monkey SV40 transformed kidney fibroblast-like cell line transfected with human FoxP4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxP4
FoxP4 (Forkhead box protein P4; also FKHLA) is a 70-80 kDa member of the winged helix transcription factor gene family, FoxP subfamily of proteins. It is a transcriptional repressor that is strongly expressed in embryonic gut and lung epithelium, and in neurons both pre-and postnatally. Postnatally, it is found in cerebellar Purkinje cells. During development, it is found in hippocampal hilus, proliferating cells of the ventricular zone, and neurons of the basolateral nucleus of the amygdala. Human FoxP4 is 680 amino acids (aa) in length. It contains a large poly-Gln region (aa 65-219), a C2H2 Zn-finger domain (aa 307-332), a Leu-zipper dimerization motift (aa 349-370) and a forkhead DNA binding domain (aa 467-559). Phosphorylation occurs at Ser360. FoxP4 forms homodimers, and heterodimers with FoxP1 and FoxP2; it does not bind CTBP1. One potential splice form shows a deletion of aa 384-395. Over aa 549-680, human and mouse FoxP4 share 89% aa identity.
Long Name
Forkhead Box P4
Alternate Names
FKHLA
Gene Symbol
FOXP4
UniProt
Additional FoxP4 Products
Product Documents for Human FoxP4 Antibody
Product Specific Notices for Human FoxP4 Antibody
For research use only
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