U87-MG human glioblastoma cell line was stained with Mouse Anti-Human FPRP/PTGFRN Monoclonal Antibody (Catalog # MAB100431, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human FPRP/PTGFRN Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: U87-MG human glioblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP/PTGFRN

FPRP (F_{2 alpha}Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha} receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton.

Long Name

Prostaglandin F2-alpha Receptor Regulatory Protein

Alternate Names

CD315, CD9P-1, PTGFRN

Entrez Gene IDs

5738 (Human); 19221 (Mouse); 29602 (Rat)

Gene Symbol

PTGFRN

UniProt

Q9P2B2 (Human)

Additional FPRP/PTGFRN Products

Product Documents for Human FPRP/PTGFRN Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FPRP/PTGFRN Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FPRP/PTGFRN Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB100431

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all FPRP/PTGFRN Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FPRP/PTGFRN Antibody

Detection of FPRP/PTGFRN in U87-MG Human Cell Line by Flow Cytometry