Human FRA-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4935
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 496106
Product Specifications
Immunogen
E. coli-derived recombinant human FRA-1
Gly146-Ala247
Accession # P15407
Gly146-Ala247
Accession # P15407
Specificity
Detects human FRA-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FRA-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FRA-1 Antibody
Detection of Human FRA‑1 by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 18 hours. PVDF Membrane was probed with 1 µg/mL of Human FRA-1 Monoclonal Antibody (Catalog # MAB4935) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRA-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human FRA-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A172 human glioblastoma cell line treated with Recombiant Human EGF (Catalog # 236-EG)
Sample: A172 human glioblastoma cell line treated with Recombiant Human EGF (Catalog # 236-EG)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FRA-1
FRA-1 (FOSL1) is a 271 amino acid member of the leucine zipper Fos family of transcription factors. Fos family members have been associated with cellular proliferation, differentiation and transformation. In accordance with these findings, overexpression of FRA-1 correlates with a malignant phenotype in breast cancer. In lung epithelia cells, FRA-1 promotes motility, invasion, and anchorage independent growth. FRA-1 knock out mice rescued for the FRA-1 placental defect are viable but show reduced bone formation that may be due to a defect in the osteoblast differentiation. Human FRA-1 and mouse FRA-1 are 94% identical between amino acids 146-247.
Long Name
Fos-related antigen 1
Alternate Names
FOSL1, FRA1
Gene Symbol
FOSL1
UniProt
Additional FRA-1 Products
Product Documents for Human FRA-1 Antibody
Product Specific Notices for Human FRA-1 Antibody
For research use only
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