Human FTO Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7208
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 729722
Product Specifications
Immunogen
E. coli-derived recombinant human FTO
Met1-Arg178
Accession # Q9C0B1
Met1-Arg178
Accession # Q9C0B1
Specificity
Detects human FTO in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human FTO Antibody
Detection of Human FTO by Western Blot.
Western blot shows lysates of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FTO Monoclonal Antibody (Catalog # MAB7208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FTO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human FTO Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with Recombinant Human FTO, see our available Western blot detection antibodies.
Sample: Cell lysates spiked with Recombinant Human FTO, see our available Western blot detection antibodies.
Western Blot
2 µg/mL
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FTO
FTO (FATSO; also known as Fat Mass and Obesity Associated gene) is a 58 kDa member of the AlkB family of Ferrous/2-Oxoglutarate-dependent oxidative DNA/RNA demethylases. It is found the nucleus, and apparently serves to protect single stranded RNA and DNA against methylation. Although FTO is ubiquitously expressed, it has high levels in neurons that are involved in regulating energy intake, and is often found to colocalize with Oxytocin. Evidence suggests it promotes feeding behavior. Human FTO is 505 amino acids (aa) in length. It contains one dioxygenase catalytic region (aa 32-327) and a C-terminal FTO domain (aa 329-500). There are three potential isoform variants. One shows a ten aa substitution for aa 1-455, a second contains a 35 aa substitution for aa 1-413, and a third utilizes an alternative start site at Met400. Over aa 1-178, human FTO shares 89% aa identity with mouse FTO.
Long Name
Fat Mass and Obesity Associated
Alternate Names
Alpha-ketoglutarate-dependent dioxygenase FTO
Gene Symbol
FTO
UniProt
Additional FTO Products
Product Documents for Human FTO Antibody
Product Specific Notices for Human FTO Antibody
For research use only
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