Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human FYB/ADAP/SLAP130 Monoclonal Antibody (Catalog # MAB38571, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Applications for Human FYB/ADAP/SLAP130 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FYB/ADAP/SLAP130

Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130 kDa (SLAP130), also known as Fyb-120 and ADAP, is an 86 kDa, 783 amino acid, cytoplasmic protein that runs anomalously as a 120‑130 kDa protein in SDS-PAGE. Human SLAP130 is a hematopoietic-specific adaptor that associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76 kDa (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known alternatively-spliced SLAP130 isoform which contains an additional 46 aa. Over the region used as immunogen, SLAP130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.

Long Name

FYN Binding Protein (FYB-120/130)

Alternate Names

ADAP, PRO0823, SLAP130

Entrez Gene IDs

2533 (Human); 23880 (Mouse); 499537 (Rat)

Gene Symbol

FYB1

UniProt

O15117

Additional FYB/ADAP/SLAP130 Products

Product Documents for Human FYB/ADAP/SLAP130 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FYB/ADAP/SLAP130 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FYB/ADAP/SLAP130 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB38571

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FYB/ADAP/SLAP130 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FYB/ADAP/SLAP130 Antibody

Detection of FYB/ADAP/SLAP130 in Jurkat Human Cell Line by Flow Cytometry.