Human GAD2/GAD65 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2247
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Bioassay, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GAD2/GAD65
Ala1-Ala150
Accession # Q05329
Ala1-Ala150
Accession # Q05329
Specificity
Detects human GAD2/GAD65 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human GAD1/GAD67 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human GAD2/GAD65 Antibody
GAD2/GAD65 in Human Dorsal Root Ganglia.
GAD2/GAD65 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using Goat Anti-Human GAD2/GAD65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2247) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human GAD2/GAD65 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human dorsal root ganglia
Sample: Immersion fixed paraffin-embedded sections of human dorsal root ganglia
Western Blot
0.1 µg/mL
Sample: Recombinant Human GAD2/GAD65
Sample: Recombinant Human GAD2/GAD65
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF2247 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GAD2/GAD65
Long Name
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)
Alternate Names
GAD65
Entrez Gene IDs
2572 (Human)
Gene Symbol
GAD2
UniProt
Additional GAD2/GAD65 Products
Product Documents for Human GAD2/GAD65 Antibody
Product Specific Notices for Human GAD2/GAD65 Antibody
For research use only
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