HEK293 human embryonic kidney cell line transfected with either (A) human Galanin R1/GALR1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Galanin R1/GALR1 Monoclonal Antibody (Catalog # MAB10353) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Galanin R1/GALR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Galanin R1/GALR1 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galanin R1/GALR1

The neuropeptide galanin elicits a range of biological effects by interaction with specific G protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.

Long Name

Galanin Receptor Type 1

Alternate Names

GAL1, GalaninR1, GALNR1, GALR1

Entrez Gene IDs

2587 (Human); 14427 (Mouse); 50577 (Rat)

Gene Symbol

GALR1

UniProt

P47211

Additional Galanin R1/GALR1 Products

Product Documents for Human Galanin R1/GALR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Galanin R1/GALR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Galanin R1/GALR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10353

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Galanin R1/GALR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Galanin R1/GALR1 Antibody

Detection of Galanin R1/GALR1 in HEK293 Human Cell Line Transfected with Human Galanin R1/GALR1 and eGFP by Flow Cytometry.