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Human Galectin-14 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5744

R&D Systems, part of Bio-Techne
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MAB5744
MAB5744-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 511124

Product Specifications

Immunogen

E. coli-derived recombinant human Galectin-14
Met1-Asp139
Accession # Q8TCE9

Specificity

Detects human Galectin-14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2, -3, -4, -7, -8, -10, or recombinant mouse Galectin-9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Galectin-14 Antibody

Detection of Human Galectin-14 antibody by Western Blot.

Detection of Human Galectin-14 by Western Blot.

Western blot shows lysates of human eosinophil. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin-14 Monoclonal Antibody (Catalog # MAB5744) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin-14 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Galectin-14 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human eosinophil

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-14

Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation.

Alternate Names

CLC2, Galectin14, LGALS14, PPL13

Entrez Gene IDs

56891 (Human)

Gene Symbol

LGALS14

UniProt

Additional Galectin-14 Products

Product Documents for Human Galectin-14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Galectin-14 Antibody

For research use only

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