Human GATA-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2606
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 532020
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694
Met27-Phe211
Accession # P43694
Specificity
Detects human GATA-4 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GATA-4 Antibody
Detection of Human GATA-4 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue . PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GATA-4 Monoclonal Antibody (Catalog # MAB2606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GATA-4 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human GATA-4 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and human liver tissue
Sample: HepG2 human hepatocellular carcinoma cell line and human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB2606 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-4
Additional GATA-4 Products
Product Documents for Human GATA-4 Antibody
Product Specific Notices for Human GATA-4 Antibody
For research use only
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