Human GCHFR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7918
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 846011
Product Specifications
Immunogen
E. coli-derived recombinant human GCHFR
Met1-Glu84
Accession # P30047
Met1-Glu84
Accession # P30047
Specificity
Detects human GCHFR in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GCHFR Antibody
Detection of Human and Mouse GCHFR by Western Blot.
Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCHFR at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GCHFR by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for GCHFR at approximately 11 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human GCHFR Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line
Sample: Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GCHFR
Long Name
GTP Cyclohydrolase I Feedback Regulator
Alternate Names
GFRP, HsT16933, p35
Gene Symbol
GCHFR
UniProt
Additional GCHFR Products
Product Documents for Human GCHFR Antibody
Product Specific Notices for Human GCHFR Antibody
For research use only
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