Human GPER/GPR30 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5534
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 730737
Product Specifications
Immunogen
E. coli-derived recombinant human GPER
Met1-Ser62
Accession # Q99527
Met1-Ser62
Accession # Q99527
Specificity
Detects human GPER in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GPER/GPR30 Antibody
GPER in Human Brain.
GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human GPER Monoclonal Antibody (Catalog # MAB5534) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human GPER/GPR30 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPER/GPR30
GPER (G-Protein Coupled Estrogen Receptor 1; also GPR30, DRY12 and mER) is a 44 kDa, seven transmembrane (TM) member of the GPR-1 family of molecules. It is expressed on/in neurons, monocytes and endothelial cells. Its exact location is unclear; it has been described in both the cell membrane and ER. Human GPER is 375 amino acids (aa) in length. It contains an N-terminal extracellular region (aa 1‑62), a series of seven TM domains (aa 63‑327), and a C-terminal cytoplasmic tail (aa 328‑375). The initial function attributed to GPER was that of a membrane receptor for estrogen. There are two potential splice variants for GPER. One shows a deletion of aa 32-49, while a second shows a 99 aa substitution for aa 308‑375. Over aa 1‑62, human GPER shares 57% aa identity with mouse GPER.
Long Name
G Protein-coupled Estrogen Receptor 1
Alternate Names
CEPR, CMKRL2, DRY12, FEG-1, GPCR-BR, GPR30, LERGU2, LyGPR
Gene Symbol
GPER1
UniProt
Additional GPER/GPR30 Products
Product Documents for Human GPER/GPR30 Antibody
Product Specific Notices for Human GPER/GPR30 Antibody
For research use only
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