Human GPR101 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7495
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # 909603
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human GPR101
Met1-Pro508,
Accession # NP_473362
Met1-Pro508,
Accession # NP_473362
Specificity
Detects human GPR101 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for Human GPR101 Antibody
Detection of Human GPR101 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line both non-transfected and transfected with human GPR101. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GPR101 at approximately 80-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of GPR101 in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP was stained with either (A) Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).Applications for Human GPR101 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human GPR101
Sample: HEK293 human embryonic kidney cell line transfected with human GPR101
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR101
Long Name
G Protein-coupled Receptor 101
Alternate Names
GPCR6
Gene Symbol
GPR101
UniProt
Additional GPR101 Products
Product Documents for Human GPR101 Antibody
Product Specific Notices for Human GPR101 Antibody
For research use only
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