Human GPR115 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5437
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 527003
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GPR115
Ser22-Ala347
Accession # Q8IZF3
Ser22-Ala347
Accession # Q8IZF3
Specificity
Detects human GPR115 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human GPR30, 49, 56, 111, 114, 124, or 125 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GPR115 Antibody
Detection of Human GPR115 by Western Blot.
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human GPR115 Monoclonal Antibody (Catalog # MAB5437) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GPR115 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human GPR115 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC)
Sample: Human peripheral blood mononuclear cells (PBMC)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR115
GPR115 is a member of the LN-7TM family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a long extracellular N-terminus (1, 2). The 695 amino acid (aa) human GPR115 sequence predicts a 21 aa signal sequence, a 385 aa N‑terminal extracellular domain (ECD), seven TM regions separated by 6‑24 aa intracellular and extracellular regions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECD contains a highly glycosylated mucin‑like stalk that is predicted to function in adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavage site (GPS) (1). GPS domains, which have been described in other 7TM proteins including ETL, GPR126, HE6, and Latrophilin-1, are cleavage sites for processing proteins into two subunits (3‑7). Within the N‑terminal region that ends with the predicted cleavage site (aa 22‑347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract (1).
References
- Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
- Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
- Nechiporuk, T. et al. (2001) J. Biol. Chem. 276:4150.
- Moriguchi, T. et al. (2004) Genes Cells 9:549.
- Kierszenbaum, A.L. (2003) Mol. Reprod. Dev. 64:1.
- Krasnoperov, V.G. et al. (1997) Neuron 18:925.
- Krasnoperov, V. et al. (2002) J. Biol. Chem. 277:46518.
Long Name
G Protein-coupled Receptor 115
Alternate Names
PGR18
Gene Symbol
ADGRF4
UniProt
Additional GPR115 Products
Product Documents for Human GPR115 Antibody
Product Specific Notices for Human GPR115 Antibody
For research use only
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