Human GPR37 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4450
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 436923
Product Summary for Human GPR37 Antibody
Immunogen
NS0 mouse myeloma cell line transfected with human GPR37
Ala27-Cys613
Accession # O15354
Ala27-Cys613
Accession # O15354
Specificity
Detects human GPR37.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human GPR37 Antibody
Detection of GPR37 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR37 Monoclonal Antibody (Catalog # MAB4450, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human GPR37 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR37
GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 613 aa, ~55 kDa orphan 7-transmembrane receptor. It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus. It is a substrate of the E3 ubiquitin ligase, parkin, which is upregulated during endoplasmic reticulum stress. In a juvenile form of Parkinson’s disease, GPR37 accumulates, contributing to stress-induced neuronal cell death. The extracellular portions of human and mouse GPR37 share 68% aa identity.
Long Name
G Protein-coupled Receptor 37
Alternate Names
ETBR-LP-1, PAELR
Gene Symbol
GPR37
UniProt
Additional GPR37 Products
Product Documents for Human GPR37 Antibody
Product Specific Notices for Human GPR37 Antibody
For research use only
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