Human GPR37L1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4449
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 444532
Product Summary for Human GPR37L1 Antibody
Immunogen
NS0 mouse myeloma cell line transfected with human GPR37L1
Accession # O60883
Accession # O60883
Specificity
Detects human GPR37L1. Stains human GPR37L1 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human GPR37L1 Antibody
Detection of GPR37L1 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR37L1 Monoclonal Antibody (Catalog # MAB4449, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human GPR37L1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR37L1
GPR37L1 (G-protein coupled receptor 37-like 1), also called ETBR-LP-2 (endothelin B receptor-like protein 2), is a 481 aa, orphan 7-transmembrane receptor with calculated molecular weight of 53 kDa. GPR37L1 is expressed in cerebellar Bergmann glia, cerebral cortex and internal capsule fibers in the central nervous system. It shows 39% aa identity with GPR37, a neuronal protein that accumulates in juvenile Parkinson’s disease neurons under endoplasmic reticulum stress. GPR37L1 does not bind known endothelin B family members. The extracellular portions of human and mouse GPR37L1 share 85% aa identity.
Long Name
G Protein-coupled Receptor 37-like 1
Alternate Names
ETBR-LP-2
Gene Symbol
GPR37L1
UniProt
Additional GPR37L1 Products
Product Documents for Human GPR37L1 Antibody
Product Specific Notices for Human GPR37L1 Antibody
For research use only
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