HEK293 human embryonic kidney cell line transfected with (A) human GPR75 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR75 Monoclonal Antibody (Catalog # MAB67281) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human GPR75 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human GPR75 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR75

GPR75 is a 540 amino acid, 7-transmembrane G-protein-coupled glycoprotein receptor mainly expressed in retinal pigment epithelial cells and brain. Polymorphisms in humans have been associated with macular degeneration. GPR75 has been identified as a receptor for CCL5/RANTES in the brain. Collectively, the extracellular domains of human GPR75 share 75% amino acid sequence identity with the corresponding regions of mouse and rat GPR75.

Long Name

G Protein-coupled Receptor 75

Alternate Names

GPRchr2

Entrez Gene IDs

10936 (Human); 237716 (Mouse); 498434 (Rat)

Gene Symbol

GPR75

UniProt

O95800

Additional GPR75 Products

Product Documents for Human GPR75 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR75 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPR75 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB67281

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GPR75 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPR75 Antibody

Detection of GPR75 in HEK293 Human Cell Line Transfected with Human GPR75 and eGFP by Flow Cytometry.