HEK293 human embryonic kidney cell line transfected with (A) GPRC5B or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human GPRC5B Monoclonal Antibody (Catalog # MAB10253) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GPRC5B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5B

G-protein coupled receptor family C group 5-member B (GPRC5B) is a retinoic acid-inducible protein that belongs to the seven-transmembrane domain family of receptors. Human GPRC5B shares approximately 85% aa sequence identity with mouse GPRC5B.

Although GPRC5B is considered an "orphan receptor" and its molecular function is not completely understood, it has been implicated in obesity-associated inflammatory signaling in adipocytes and in diet-induced insulin resistance. Consistent with that, CRISPR-Cas9-mediated knock-down of the GPRC5B gene allowed the identification of signaling pathways linking the GPRC5B receptor expression to beta-cell proliferation and apoptosis.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member B

Alternate Names

RAIG2

Entrez Gene IDs

51704 (Human); 64297 (Mouse); 293546 (Rat)

Gene Symbol

GPRC5B

UniProt

Q9NZH0

Additional GPRC5B Products

Product Documents for Human GPRC5B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPRC5B Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPRC5B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10253

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GPRC5B Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPRC5B Antibody

Detection of GPRC5B in HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP by Flow Cytometry.