Human Hemopexin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4490
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 698813
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hemopexin
Thr24-His462
Accession # P02790
Thr24-His462
Accession # P02790
Specificity
Detects human Hemopexin in direct ELISAs and Western blots.
In Western blots, approximately 5-10%
cross-reactivity with recombinant human MMP-1, -3, -10, -12, and -13 is
observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Hemopexin Antibody
Detection of Human Hemopexin by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Hemopexin Monoclonal Antibody (Catalog # MAB4490) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hemopexin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.Hemopexin in Human Liver.
Hemopexin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Hemopexin Monoclonal Antibody (Catalog # MAB4490) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Hemopexin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Western Blot
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hemopexin
References
- Tolosano, E. and Altruda, F. (2002) DNA and Cell Biol. 21:297.
- Mauk, M. R. et al. (2007) Nature Pro. Rep. 24:523.
- Ascenzi, P. et al. (2005) IUMB Life. 57:749.
Alternate Names
Beta-1B-glycoprotein, HPX
Gene Symbol
HPX
UniProt
Additional Hemopexin Products
Product Documents for Human Hemopexin Antibody
Product Specific Notices for Human Hemopexin Antibody
For research use only
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