Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2710

R&D Systems, part of Bio-Techne
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AF2710
AF2710-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2
Gln179-Arg605
Accession # Q96MM7

Specificity

Detects human Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human HS6ST2, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Heparan Sulfate6-O-Sulfotransferase 2/HS6ST2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2

HS6ST2 is a key enzyme in HS biosynthesis. There are at least three alternatively spliced forms, which include a long form (645 aa, NP_001070656), an intermediate form (605 aa, Q96MM7) with 40 aa deleted between Arg 316 and Asn 357 of the long form, and a short form starting at Met 147 of the intermediate form. Both the long and intermediate forms predict a type II membrane protein, consistent with its Golgi location. The long form of human HS6ST2 shares 94% and 86% aa sequence identities with that of chimpanzee, dog, mouse, rat, and chicken, respectively.

Alternate Names

EC-1

Entrez Gene IDs

90161 (Human)

Gene Symbol

HS6ST2

UniProt

Q96MM7

Additional Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody

For research use only

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