Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1052939

View all Hepassocin/FGL1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690

Specificity

Detects human Hepassocin/FGL1 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Hepassocin/FGL1 Antibody

Detection of Hepassocin/FGL1 in HEK293 cells transfected with Human Hepassocin and eGFP cells by Flow Cytometry.

HEK293 cells transfected with Human Hepassocin and eGFP were stained with eGFP and either (A) Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16143) or (B) Mouse IgG2B Isotype Control (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.

Detection of Human Hepassocin/FGL1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Human Liver. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Hepassocin/FGL1 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 cells transfected with Human Hepassocin and eGFP.

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line and Human Liver.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and FGL1, is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. Hepassocin may play a role in the development of hepatocellular carcinomas.

Long Name

Fibrinogen Like 1

Alternate Names

FGL1, Fibrinogen-like-protein 1, FREP1

Entrez Gene IDs

2267 (Human); 234199 (Mouse); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Gene Symbol

FGL1

UniProt

BAB70690

Additional Hepassocin/FGL1 Products

Product Documents for Human Hepassocin/FGL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Hepassocin/FGL1 Antibody

For research use only

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