Human Hepsin Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4776
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepsin
Arg45-Leu417 (Asp161Glu, Arg162Lys)
Accession # P05981
Arg45-Leu417 (Asp161Glu, Arg162Lys)
Accession # P05981
Specificity
Detects human Hepsin in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Hepsin Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Hepsin (Catalog # 4776-SE)
Sample: Recombinant Human Hepsin (Catalog # 4776-SE)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hepsin
Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6).
References
- Leytus, S.P. et al. (1988) Biochemistry 27:1067.
- Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
- Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
- Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
- Dhanasekaran, S.M. et al. (2001) Nature 412:822.
- Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.
Alternate Names
HPN, TMPRSS1
Gene Symbol
HPN
UniProt
Additional Hepsin Products
Product Documents for Human Hepsin Biotinylated Antibody
Product Specific Notices for Human Hepsin Biotinylated Antibody
For research use only
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