Human HES-4 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3600
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HES-4
Val94-Arg221
Accession # Q9HCC6
Val94-Arg221
Accession # Q9HCC6
Specificity
Detects human HES-4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) HES-1, rhHES-2, rhHES-5, and rhHES-7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human HES-4 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human HES-4
Sample: Recombinant Human HES-4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HES-4
HES-4 is a 221 aa, 24 kDa member of the HES-family of transcriptional repressors. HES-4 contains a species-specific bHLH domain (aa 33‑93), a specific orange DNA-binding domain (aa 109‑150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C-terminal motif. HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency. In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical.
Long Name
Hairy And Enhancer of Split 4
Alternate Names
HES4
Entrez Gene IDs
57801 (Human)
Gene Symbol
HES4
UniProt
Additional HES-4 Products
Product Documents for Human HES-4 Biotinylated Antibody
Product Specific Notices for Human HES-4 Biotinylated Antibody
For research use only
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