Human HM74A/PUMA-G/GPR109A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB27601
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 245115
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human HM74A/PUMA-G/GPR109A
Met1-Pro363
Accession # Q8TDS4
Met1-Pro363
Accession # Q8TDS4
Specificity
Detects human HM74A/PUMA-G/GPR109A in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human HM74A/PUMA-G/GPR109A Antibody
HM74A/PUMA-G/GPR109A in Human Spleen Tissue.
HM74A/PUMA-G/GPR109A was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB27601) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human HM74A/PUMA-G/GPR109A Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen tissue
Sample: Immersion fixed paraffin-embedded sections of human spleen tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HM74A/PUMA-G/GPR109A
HM74A, also known as GPR109A, is a high affinity G-protein coupled receptor for nicotinic acid. It is primarily expressed in adipose tissue and spleen.
Long Name
G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A
Alternate Names
GPR109A, HCA2, HCAR2, Niacr1, Nicotinic Acid Receptor, PUMAG
Gene Symbol
HCAR2
UniProt
Additional HM74A/PUMA-G/GPR109A Products
Product Documents for Human HM74A/PUMA-G/GPR109A Antibody
Product Specific Notices for Human HM74A/PUMA-G/GPR109A Antibody
For research use only
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