Human HOXD10 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF10413

R&D Systems, part of Bio-Techne
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AF10413-100
AF10413-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all HOXD10 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HOXD10
Arg153-Thr262
Accession # P28358

Specificity

Detects human HOXD10 in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human HOXD10 Antibody

HOXD10 antibody in Human iPS Cells Differentiated to NPCs by Immunocytochemistry (ICC).

HOXD10 in Human iPS Cells Differentiated to NPCs.

HOXD10 was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to neural progenitor cells (NPC) using Goat Anti-Human HOXD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10413) at 10 µg/mL, and co-stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259) at 10 µg/mL, for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001), NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining for HOXD10 was localized to nucleus, while Nestin co-staining was cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human HOXD10 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human induced pluripotent stem (iPS) cells differentiated to neural progenitor cells (NPC)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOXD10

HOXD10 is a sequence-specific transcription factor that belongs to the Abd-B homeobox family.  It is a nuclear protein that is expressed in developing limb buds and is involved in differentiation and limb development.  HOXD10 mutations have been associated with foot deformities such as rocker-bottom foot and the deformity seen in Charcot-Marie-Tooth disease.  HOXD10 also functions as a candidate tumor suppressor.  In gastric cancer there is downregulation in gastric cancer tissues and cell lines relative to normal stomach tissues.  It has also been reported to suppress ovarian cancer, hepatocellular carcinoma and other cancer cells. Re-expression of HOXD10 results in significant inhibition of cell survival, induction of cell apoptosis and impairment of cell migration and invasion.

Long Name

Homeobox D10

Alternate Names

HOX4, HOX4D, HOX4E

Entrez Gene IDs

3236 (Human)

Gene Symbol

HOXD10

UniProt

P28358

Additional HOXD10 Products

Product Documents for Human HOXD10 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HOXD10 Antibody

For research use only

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