Human ICA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4084
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ICA1
Ser2-Ala482
Accession # ABW03414
Ser2-Ala482
Accession # ABW03414
Specificity
Detects human ICA1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human ICA1 Antibody
Detection of ICA1 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084, filled histo-gram) or isotype control antibody (AB-108-C, open histo-gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
ICA1 in paraffin-embedded human pancreas.
ICA1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (red; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to islets of Langerhans.Applications for Human ICA1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.1 µg/mL
Sample: Recombinant Human ICA1
Sample: Recombinant Human ICA1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICA1
Islet Cell Autoantigen 1 (ICA1), also known as ICA 69 kDa (ICA69), is a cytosolic protein that is associated with the Golgi complex and with immature secretory granules. Its N-terminal region shares similarity with arfaptins (ADP-ribosylation factor-interacting-proteins), suggesting that ICA1 likely plays a role in membrane trafficking. Human ICA-1 is a 483 amino acids (aa) protein. As a result of aa insertions, deletions and substitutions, multiple isoforms exist. Human ICA-1 shows 89% and 94% aa identity to mouse and canine ICA-1, respectively.
Long Name
Islet Cell Autoantigen 1
Alternate Names
ICAp69
Entrez Gene IDs
3382 (Human)
Gene Symbol
ICA1
UniProt
Additional ICA1 Products
Product Documents for Human ICA1 Antibody
Product Specific Notices for Human ICA1 Antibody
For research use only
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