Human ICAM-3/CD50 Antibody
R&D Systems, part of Bio-Techne | Catalog # BBA29
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Adhesion Blockade, CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Adhesion Assay, Direct ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # Cal 3.10
Product Specifications
Immunogen
Recombinant human ICAM-3/CD50 Fc Chimera
Specificity
Detects human ICAM-3/CD50 in Western blots. Does not cross-react with recombinant human (rh) ICAM-1 or rhVCAM-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human ICAM-3/CD50 Antibody
Application
Recommended Usage
Adhesion Blockade
Bossy, D. et al. (1995) Eur. J. Immunol. 25:459.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood neutrophils
Sample: Human peripheral blood neutrophils
Western Blot
1 µg/mL
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-3/CD50
Intercellular Adhesion Molecule-3 (ICAM-3), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alphad/ beta2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in mice.
References
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Entrez Gene IDs
3385 (Human)
Gene Symbol
ICAM3
Additional ICAM-3/CD50 Products
Product Documents for Human ICAM-3/CD50 Antibody
Product Specific Notices for Human ICAM-3/CD50 Antibody
For research use only
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