Human ICAM-3/CD50 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF715
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM‑3/CD50
Specificity
Detects human ICAM‑3/CD50 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ICAM-1 is observed and less than 1% cross-reactivity with recombinant mouse (rm) ICAM-1, recombinant rat ICAM-1, rhICAM-2, rhVCAM-1, and rmVCAM-1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ICAM-3/CD50 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-3/CD50
Intercellular Adhesion Molecule‑3 (ICAM‑3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA‑1 (CD11a/CD18) and alphad/ beta2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM‑3 has not been identified in the mouse.
References
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Entrez Gene IDs
3385 (Human)
Gene Symbol
ICAM3
Additional ICAM-3/CD50 Products
Product Documents for Human ICAM-3/CD50 Antibody
Product Specific Notices for Human ICAM-3/CD50 Antibody
For research use only
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