Human IFN-alpha 6/IFNA6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10212
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1009720
Product Specifications
Immunogen
E. coli-derived recombinant human IFN-alpha 6/IFNA6
Leu28-Lys207
Accession # Q90W0
Leu28-Lys207
Accession # Q90W0
Specificity
Detects human IFN-alpha 6/IFNA6 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human IFN-alpha 6/IFNA6 Antibody
Detection of Human IFN-alpha 6/IFNA6 by Western Blot.
Western blot shows lysates of HEK001 human epidermal keratinocyte cell line and NHEK human normal epidermal keratinocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IFN-alpha 6/IFNA6 Monoclonal Antibody (Catalog # MAB10212) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IFN-alpha 6/IFNA6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IFN-alpha 6/IFNA6 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HEK001 human epidermal keratinocyte cell line and NHEK human normal epidermal keratinocytes
Sample: HEK001 human epidermal keratinocyte cell line and NHEK human normal epidermal keratinocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha 6/IFNA6
Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.
Long Name
Interferon alpha 6
Alternate Names
IFN-alpha K, IFNA6, IFNalpha K
Gene Symbol
IFNA6
UniProt
Additional IFN-alpha 6/IFNA6 Products
Product Documents for Human IFN-alpha 6/IFNA6 Antibody
Product Specific Notices for Human IFN-alpha 6/IFNA6 Antibody
For research use only
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