Human IFN-alpha I Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10100
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 995842
Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human IFN‑ alphaI
Met1-Asp189
Accession # P01571
Met1-Asp189
Accession # P01571
Specificity
Detects human IFN‑ alphaI, - alpha2, -4 alpha, -5, -6, -7, -10, -16, -17,and -21 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IFN-alpha I Antibody
IFN-alpha I Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-alpha I Antibody.
Recombinant Human IFN-alpha I reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-alpha I (20 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN-alpha I Monoclonal Antibody (Catalog # MAB10100). The ND50 is typically 1.5-15 ng/mL.Applications for Human IFN-alpha I Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IFN‑ alphaI inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 1.5-15 ng/mL in the presence of 20 pg/mL Recombinant Human IFN‑ alphaI.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha I
Interferon-alpha (IFN-alpha, IFNA), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. There are at least 18 different IFNA subtypes, and the major subtypes identified are IFNA1, IFNA2, IFNA8, IFNA10, IFNA14 and IFNA21.
Long Name
Interferon alpha I
Alternate Names
IFNA17, IFNalpha I
Entrez Gene IDs
3451 (Human)
Gene Symbol
IFNA17
UniProt
Additional IFN-alpha I Products
Product Documents for Human IFN-alpha I Antibody
Product Specific Notices for Human IFN-alpha I Antibody
For research use only
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