Human IGFBP-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF804
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Papio cynocephalus (Yellow Baboon)
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP-4
Asp22-Glu258
Accession # AAA62670
Asp22-Glu258
Accession # AAA62670
Specificity
Detects human IGFBP‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGFBP-4 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-5 and rhIGFBP-6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IGFBP-4 Antibody
IGFBP‑4 in Human Placenta.
IGFBP-4 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 5 µg/mL Goat Anti-Human IGFBP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
IGFBP‑4 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑4 Antibody.
Recombinant Human IGFBP-4 (Catalog # 804-GB) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-4 (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804). The ND50 is typically 12-60 µg/mL.
Detection of Human IGFBP-4 by Immunohistochemistry
Immunoreactivity (red colour) against IGFBP-4, −5, and PAPPA2 in the syncytiotrophoblast of first trimester placental villi. Serial cross sections of placental villi at 5 and 6 weeks of gestation (7 and 13 weeks not shown) were stained for IGFBP-4, IGFBP-5, and PAPP-A2. Negative controls used non-specific goat IgG in the place of the primary antibodies. FB = fetal blood vessel, CM = chorionic mesoderm, ST = syncytiotrophoblast. Scale bars denote 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25475528), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human IGFBP-4 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
0.1 µg/mL
Sample: Recombinant Human IGFBP-4 (Catalog # 804-GB)
Sample: Recombinant Human IGFBP-4 (Catalog # 804-GB)
Neutralization
Measured by its ability to neutralize IGFBP‑4 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 12-60 µg/mL in the presence of 0.3 μg/mL Recombinant Human IGFBP‑4 and 14 ng/mL Recombinant Human IGF‑II.
Reviewed Applications
Read 1 review rated 3 using AF804 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-4
Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is processed to generate the 237 aa residue mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both the mouse and rat IGFBP-4.
According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins
(IGFBP-1, -2, -3, ‑4, ‑5, ‑6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino- and carboxy-terminal third of the molecule. IGFBPs have been shown to either inhibit or enhance the biological activities of IGF, or act in an IGF-independent manner. Post-translational modification of IGFBPs, including phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions.
References
- Jones, J. I. and D.R. Clemons (1995) Endocrine Rev. 16:3.
- Kelly, K.M. et al. (1996) Intl. J. Biochem. Cell Biol. 28:619.
- Kim, H-S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12981.
Long Name
Insulin-like Growth Factor Binding Protein 4
Alternate Names
IGFBP4
Gene Symbol
IGFBP4
UniProt
Additional IGFBP-4 Products
Product Documents for Human IGFBP-4 Antibody
Product Specific Notices for Human IGFBP-4 Antibody
For research use only
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