Human IGFL-3 Antibody

Name: Human IGFL-3 Antibody
Brand: R&D Systems
SKU: MAB5970

R&D Systems, part of Bio-Techne | Catalog # MAB5970

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 536825

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Product Specifications

Immunogen

E. coli-derived recombinant human IGFL-3
Thr25-Pro125
Accession # Q6UXB1

Specificity

Detects human IGFL-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IGF‑I, rhIGF-II, rhIGFL-1, rhIGFL-4, and recombinant mouse IGFL-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human IGFL-3 Antibody

Detection of Human IGFL-3 by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGFL-3 Monoclonal Antibody (Catalog # MAB5970) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGFL-3 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human IGFL-3 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Western Blot

2 µg/mL
Sample: Human heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFL-3

IGFL-3 (insulin-like growth factor-like 3) is one of four small (~11 kDa), probably secreted human IGFL family members. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family. Mature IGFL-3 is a 101 amino acid (aa) protein that shares 41% aa identity with the sole mouse IGFL. RT-PCR data indicates its expression within the cerebellum.

Long Name

IGF-like Family Member 3

Alternate Names

IGFL3

Entrez Gene IDs

388555 (Human)

Gene Symbol