Human Intelectin-1/Omentin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4254
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 450520
Product Specifications
Immunogen
E. coli-derived recombinant human Intelectin-1/Omentin
Thr19-Ser298
Accession # Q8WWA0
Thr19-Ser298
Accession # Q8WWA0
Specificity
Detects human Intelectin-1/Omentin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Intelectin-1/Omentin Antibody
Human Intelectin‑1/Omentin ELISA Standard Curve.
Recombinant Human Intelectin-1/Omentin protein was serially diluted 2-fold and captured by Mouse Anti-Human Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB4254) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB42541) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Intelectin-1/Omentin Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Intelectin‑1/Omentin Monoclonal Antibody Catalog # MAB42541.
This product is intended for assay development on various assay platforms requiring antibody pairs.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Intelectin-1/Omentin
Intelectin-1 (Intestinal Lectin; also ITLN-1 and Omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.
Alternate Names
HL-1, Intelectin1, ITLN1, Itlna, LFR, Omentin, Omentin-1
Gene Symbol
ITLN1
UniProt
Additional Intelectin-1/Omentin Products
Product Documents for Human Intelectin-1/Omentin Antibody
Product Specific Notices for Human Intelectin-1/Omentin Antibody
For research use only
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