Human Intelectin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8004
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 874029
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Intelectin-2
Met1-Tyr324
Accession # Q8WWU7
Met1-Tyr324
Accession # Q8WWU7
Specificity
Detects human Intelectin-2 in ELISA. In Direct ELISA, no cross-reactivity with recombinant human (rh) Intelectin-1, recombinant mouse (rm) Intelectin-1, or rmIntelectin-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Intelectin-2 Antibody
Detection of Human Intelectin‑2 by Western Blot.
Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Intelectin-2 Monoclonal Antibody (Catalog # MAB8004) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Intelectin-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intelectin‑2 in Human Colon.
Intelectin-2 was detected in formalin fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human Intelectin-2 Monoclonal Antibody (Catalog # MAB8004) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Intelectin-2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Formalin fixed paraffin-embedded sections of normal human colon
Sample: Formalin fixed paraffin-embedded sections of normal human colon
Western Blot
2 µg/mL
Sample: Human small intestine tissue
Sample: Human small intestine tissue
Reviewed Applications
Read 1 review rated 5 using MAB8004 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Intelectin-2
Alternate Names
HL-2, Intelectin-b, Intelectin2, Itln1b, ITLN2, Itlnb
Gene Symbol
ITLN2
UniProt
Additional Intelectin-2 Products
Product Documents for Human Intelectin-2 Antibody
Product Specific Notices for Human Intelectin-2 Antibody
For research use only
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