Human IRAK4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3919

R&D Systems, part of Bio-Techne
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MAB3919-100
MAB3919-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 413609

View all IRAK4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRAK4
Met1-Ser460
Accession # Q9NWZ3

Specificity

Detects human IRAK4 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Human IRAK4 Antibody

IRAK4 antibody in Human Tonsil by Immunohistochemistry (IHC-P).

IRAK4 in Human Tonsil.

IRAK4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Human IRAK4 Monoclonal Antibody (Catalog # MAB3919) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human IRAK4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRAK4

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that help mediate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. IRAK4 mutations have been described in patients with recurrent bacterial infections and poor inflammatory responses.

Long Name

Interleukin-1 Receptor-Associated Kinase 4

Alternate Names

REN64

Entrez Gene IDs

51135 (Human); 266632 (Mouse); 300177 (Rat)

Gene Symbol

IRAK4

UniProt

Q9NWZ3

Additional IRAK4 Products

Product Documents for Human IRAK4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRAK4 Antibody

For research use only

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